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Upcoming workshop · 1-Month Bioinformatics Training

From Linux Fundamentals to a Hands-On RNA-Seq Project

A 4-week live online bioinformatics training by Discovery Boulevard

30 October – 26 November 2026 7:00 – 8:00 PM IST ₹8,500 / $120
Register now Fees & payment

About the workshop

A one-month program designed for complete beginners entering bioinformatics and transcriptomics. Every participant gets individual login credentials to a remote Linux server from the first session, and practises on real biological datasets, including publicly available large-scale sequencing data.

Over 20 live practical sessions you learn to run commands, process biological files, run bioinformatics tools and troubleshoot errors. You progress from Linux fundamentals to NCBI databases, BLAST, sequence alignment and primer design, and finish with a guided bulk RNA-Seq mini project on public sequencing data.

Sessions run live online from 7:00 to 8:00 PM IST, Monday to Friday, with live instructor guidance throughout and a dedicated WhatsApp group for queries, troubleshooting and technical help.

  • 20 live sessions
  • Remote Linux server access
  • Session recordings
  • PDF tutorials
  • Certificate of participation
1-Month Bioinformatics Training flyer

Curriculum

What the workshop covers

Four weeks, five evenings a week: from your first Linux command to a complete RNA-Seq differential expression analysis.

  1. Week 1 · 30 Oct – 5 Nov

    Linux Essentials & Remote Server Access

    Getting onto the training server and moving around the command line with confidence.

    Day 1 · Fri 30 Oct

    • Introduction to bioinformatics and Linux
    • Ubuntu, WSL, PuTTY, WinSCP and FileZilla
    • Server login and training server setup

    Day 2 · Mon 2 Nov

    • pwd, ls, cd and cd ..
    • Absolute vs relative paths

    Day 3 · Tue 3 Nov

    • touch, mkdir, rmdir, rm, rm -r and cat

    Day 4 · Wed 4 Nov

    • cp, mv and renaming files
    • Wildcards (*)

    Day 5 · Thu 5 Nov

    • File permissions and hidden files
    • Metacharacters, pipes (|) and combining commands
  2. Week 2 · 6 – 12 Nov

    Linux Commands for Real Bioinformatics Data

    Working with large FASTA, FASTQ and tabular files the way bioinformaticians do.

    Day 6 · Fri 6 Nov

    • head, tail and wc
    • Exploring large FASTA / FASTQ files

    Day 7 · Mon 9 Nov

    • cut, paste, sort and uniq
    • Processing tabular biological data

    Day 8 · Tue 10 Nov

    • grep and advanced searching
    • Regular expressions on biological datasets

    Day 9 · Wed 11 Nov

    • ps, kill and nohup
    • Background jobs and long-running tasks

    Day 10 · Thu 12 Nov

    • gzip and gunzip
    • Combining commands for efficient data processing
  3. Week 3 · 13 – 19 Nov

    Biological Databases & Sequence Analysis

    Finding sequences and literature, and comparing sequences with BLAST and alignments.

    Day 11 · Fri 13 Nov

    • Introduction to NCBI
    • Gene database searching and information retrieval

    Day 12 · Mon 16 Nov

    • NCBI Nucleotide and Protein databases
    • Sequence retrieval and FASTA files

    Day 13 · Tue 17 Nov

    • PubMed literature search
    • Exploring published biological research

    Day 14 · Wed 18 Nov

    • BLAST (blastn, blastp)
    • Score, % identity, query coverage and E-value

    Day 15 · Thu 19 Nov

    • Pairwise vs multiple sequence alignment
    • Identifying conserved regions
  4. Week 4 · 20 – 26 Nov

    Primer Design & RNA-Seq Mini Project

    Designing primers, then running a complete bulk RNA-Seq analysis on public data.

    Day 16 · Fri 20 Nov

    • Multiple alignment with Clustal Omega / MUSCLE
    • Consensus sequence identification

    Day 17 · Mon 23 Nov

    • Primer design principles
    • GC%, melting temperature (Tm), hairpins and dimers

    Day 18 · Tue 24 Nov

    • Primer design with Primer3
    • Specificity checks with NCBI Primer-BLAST

    Day 19 · Wed 25 Nov

    • The bulk RNA-Seq workflow
    • QC, trimming, alignment, read counting and differential expression

    Day 20 · Thu 26 Nov

    • Hands-on RNA-Seq mini project on public data
    • FastQC, fastp, HISAT2, SAMtools, featureCounts and DESeq2
    • PCA, heatmaps, volcano plots and biological interpretation

What you receive

  • Individual login credentials for a remote Linux server
  • Hands-on exercises on real and public sequencing datasets
  • Recorded videos of all sessions
  • Course materials and step-by-step workflow tutorials (PDF)
  • A dedicated WhatsApp group for queries and troubleshooting
  • Live instructor guidance and doubt-clearing
  • Certificate of participation

Who it is for

  • Complete beginners entering bioinformatics and transcriptomics
  • Students and researchers who want hands-on command-line skills on a real server
  • Anyone who wants to run an RNA-Seq differential expression analysis from raw data to results

Register

Fees & registration

₹8,500 / $120

Rupees for participants in India, US dollars for international participants.

  1. 1. Fill in the registration form

    Go to the form

  2. 2. Pay the workshop fee

    Pay · India (₹)Pay · International ($)Pay · International (PayPal)

    After paying, email the transaction screenshot to support@discoveryboulevard.com.

Good to know

  • • Server access duration, resource limits and usage guidelines are shared with registered participants.
  • • Dataset size and analysis scope depend on available computational resources.
  • • Server credentials are for registered participants only and must not be shared. Do not upload confidential or personal data to the server.
  • • Recordings, materials and certificates are provided to registered participants.
  • • Schedule updates are shared through the WhatsApp group.
  • • The registration fee is non-refundable and non-transferable.

Registration and queries

Registration form

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